Abstract: The best station arrangement strategy of TDOA location system based on genetic algorithm is presented to advance the location accuracy of the TDOA location system.The fitness principle to be contented is that the best station arrangement strategy makes the average CRLB of target space least.The encoding of node position and fitness function in GA are researched.On the basis of this,an algorithm to search the best station arrangement strategy is proposed based on GA.Simulation result shows that the disposal of station arrangement to this algorithm approaches to the best station arrangement strategy.